The application of the CRISPR–Cas9 system in Pseudomonas syringae pv. actinidiae
نویسندگان
چکیده
منابع مشابه
Transposon insertion libraries for the characterization of mutants from the kiwifruit pathogen Pseudomonas syringae pv. actinidiae
Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit canker, is one of the most devastating plant diseases of recent times. We have generated two mini-Tn5-based random insertion libraries of Psa ICMP 18884. The first, a 'phenotype of interest' (POI) library, consists of 10,368 independent mutants gridded into 96-well plates. By replica plating onto selective media, the POI l...
متن کاملNew insights on the bacterial canker of kiwifruit (Pseudomonas syringae pv. actinidiae)
Since 2008, Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit has become the main pathogen of yellow and green fleshed kiwifruit. All major kiwifruit producing countries in the world have been affected by this bacterial pathogen, leading to substantial economic losses. This review presents the current knowledge on various aspects about the origin, epidemiolo...
متن کاملComplete DNA Sequence of Pseudomonas syringae pv. actinidiae, the Causal Agent of Kiwifruit Canker Disease
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker of kiwifruit, a disease that has rapidly spread worldwide. We have fully sequenced and assembled the chromosomal and plasmid DNA from P. syringae pv. actinidiae ICMP 18884 using the PacBio RS II platform.
متن کاملGenome analysis of the kiwifruit canker pathogen Pseudomonas syringae pv. actinidiae biovar 5
Pseudomonas syringae pv. actinidiae (Psa) is a destructive pathogen of kiwifruit bacterial canker disease, causing severe economic losses to kiwifruit industry worldwide. Biovar 5 is the most recently reported biovar of Psa, and is found in only a local area of Japan at present. There is not much information of genetic characteristics of biovar 5. Thus, the genome of biovar 5 was sequenced and ...
متن کاملIdentification of bacteriophages for biocontrol of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae.
Pseudomonas syringae pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (Actinidia sp.). Since 2008, a global outbreak of P. syringae pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in P. syringae pv. actinidiae and other pathovars against antibiotics and copper sprays have ...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2020
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.001124